WebThe CytoSelect™ Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). WebThe phagocytic activity of monocyte-derived macrophages was studied using the CytoSelect™ phagocytosis kit (catalogue number CBA-224, Cell Biolabs, San ... T cells) in triplicate in 96-well flat-bottomed ... kit (catalogue G5421, CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay, Promega, Germany) as we previously ...
CytoSelect™ Cell Viability and Cytotoxicity Assay - Cell Biolabs
WebCytoSelect™ 96-well Phagocytosis Assay does not involve subjective manual counting of RBCs. Instead cells are lysed and detected by the proprietary erythrocyte substrate in a microtiter plate reader. This format provides a quantitative, high-throughput method to accurately measure phagocytosis. The CytoSelect™ 96-well Phagocytosis Assay ... WebMay 12, 2024 · For phagocytosis and cell lysis, we have utilized CytoSelect™ 96-Well Phagocytosis RBS substrate Assay (CBA-220, Cell Biolabs Inc, San Diego, CA). Briefly, the lytic ability of macrophages towards sheep erythrocytes was analyzed in a 96 well plate as per manufacturer's protocol with RBC to macrophage ratio maintained at 10:1. flowatch流速仪
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WebSep 10, 2024 · ( D) Phagocytic activity of mouse bone marrow neutrophils (n = 6) was assessed using a CytoSelect 96-Well Phagocytosis Assay kit (E. coli Substrate) and data were expressed as optical density (further check Material and methods, Section 4.9 ). WebJun 21, 2024 · Phagocytosis assay The SW-10 cells were plated in 96-well plates and pretreated with Cytochalasin D, an inhibitor of actin polymerization, at the designated concentration for 1 h. The cells were … Webaccurately measure phagocytosis. The CytoSelect™ 96-well Phagocytosis Assay provides a robust system for screening TLR ligands, phagocytosis activators or inhibitors. … greek culture in chicago