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Hur antibody

WebProduct Details Product Specific Information This antibody detects pS, pT and pY amino acids with very low cross reactivity to their non-phosphorylated variants Target Information There are three amino acids … Web10 feb. 2011 · Using a phospho-Ser 318-specific HuR antibody, we found a strong increase in nuclear HuR phosphorylation in DLD-1 cells when compared with nontransformed CCD 841 colon epithelial cells. Importantly, a strong increase in HuR phosphorylation at Ser 318 was also found in tissue specimen from human colon carcinomas.

HuR Posttranscriptionally Regulates WEE1: Implications for the …

Web22 dec. 2014 · The mRNA binding protein HuR is over expressed in cancer cells and contributes to disease progression through post-transcriptional regulation of mRNA. The regulation of HuR and how this relates to glioma is the focus of this report. SRC and c-Abl kinases regulate HuR sub-cellular trafficking and influence accumulation in the … Web14 mrt. 2024 · To determine the MUC16 mediated HuR target genes in TNBC, we performed RNA immunoprecipitation (RIP) with HuR antibody, followed by a tumor metastasis PCR array. First, HuR target genes were pull-down using HuR antibody from scramble control (HCC1806-SCR) cells and MUC16 knockdown (HCC1806-shMUC16) … punalakintie 9 https://headinthegutter.com

High-constitutive HuR phosphorylation at Ser 318 by PKC{delta ... - PubMed

WebImmunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody (ab196626) Immunofluorscence analysis of MCF7 cells labeling HuR / ELAVL1 with ab196626 at 1/100 dilution (top panel). Blue: DAPI for nuclear staining (bottom panel). Western blot - Anti-HuR / ELAVL1 antibody (ab196626) WebB.RIP Lysate prepared from HeLa cells (0.5 × 10 6 or 1.7 × 10 6 cell equivalents per IP) were immunoprecipated using 2.5 μg of either a normal Rabbit IgG or Anti-HUR antibody and the Imprint RNA Immunoprecipitation Kit . Immunoprecipitation of HUR-target and non-target associated RNA was validated by qPCR using Control Primers Actin B and JUN. WebUsing a phospho-Ser 318-specific HuR antibody, we found a strong increase in nuclear HuR phosphorylation in DLD-1 cells when compared with nontransformed CCD 841 colon epithelial cells. Importantly, a strong increase in HuR phosphorylation at Ser 318 was also found in tissue specimen from human colon carcinomas. punakylkirastas ääni

HuR antibody (11910-1-AP) Proteintech - ptglab

Category:Poly(ADP-ribosyl)ation enhances HuR oligomerization and contributes …

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Hur antibody

Transcript Stabilization by the RNA-Binding Protein HuR Is …

WebHuR Antibody (A-21277) in IHC Molecular and cellular biology 2024 - Published figure using HuR monoclonal antibody (Product # A-21277) in Immunohistochemistry … WebAntibody Dilution Buffer (1X PBS / 1% BSA / 0.3% Triton X-100): To prepare 10 ml, add 30 µl Triton™ X-100 to 10 ml 1X PBS. Mix well then add 0.1 g BSA , mix. Recommended …

Hur antibody

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WebELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a … WebInvitrogen Anti-HuR Monoclonal (3A2), Catalog # MA1-167. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunoprecipitation (IP) and RNA Immunoprecipitation …

Webclonal antibodies against HuR protein. Generated an-tibodies showed high specificity to HuR protein and immunoprecipitated endogenous HuR protein from Jurkat and BJAB lysates. Using these antibodies and commercial anti-HuR antibodies, the regulation of HuR expression were examined and protein profile in HuR-mRNA complexes was … Web14 sep. 2024 · To this end, HuR HepKO mice were treated with an OPN-neutralizing antibody or control antibody while being fed the 60%-HFD-CD diet. OPN blockade resulted in a tendency toward reduced fibrosis in HuR HepKO mice compared to control antibody treatment, as shown by picrosirius staining (Figure 7D,E).

WebPublished figure using HuR monoclonal antibody (Product # A-21277) in Immunohistochemistry Figure 3 Knockdown of IGF2BPs decreases mRNA stability … WebIHC was performed as previously described. 29 After incubation with anti-Cleaved Caspase-3 or anti-HuR antibodies (#12,582) overnight at 4 °C, the samples were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies for 10 min at 37 °C. All imaged were obtained using an upright microscope (BX53, Olympus, Japan).

Web31 dec. 2024 · Here, we provide evidence suggesting that HuR is involved in the TLR3-mediated antiviral innate immune response by stabilizing the mRNA of Atp6v0d2, which encodes a subunit of vacuolar-type H +...

WebHuR and polypyrimidine tract binding protein (PTB) have been described as 'global regulators' of RNA alternative splicing, stability, and translation in non-neuronal (including ectodermal) tissues examined to date in diverse species, … punalehtinen apilaWeb1 dag geleden · The following primary antibodies were used for immunoblotting: antibodies against HA Tag (3724, CST), HuR (2582, CST), and pERK1/2 (4370, CST) were used at 1:1,000 dilution, and antibodies against Vinculin (13901, CST) and Tubulin (T6199, Sigma) were used at 1:3,000 dilution as the internal controls. punalaputetut tuotteetWebApplications. This RIPAb+ HuR -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. RIP lysate from MCF7 cells (2 X 10 6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 μg of either a normal rabbit IgG or Anti-HuR antibody. punalehtiset pensaatWebDescription. HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. punalehtinen vaahteraWebCompare anti-SERPINB13 Antibody ABIN1398419 from antibodies-online on Biocompare.com. Welcome Guest. Sign In Register. Products. Popular ... NCBI Gene Aliases headpin, HUR7, HU, 5430417G24, HURPIN, HSHUR7SEQ, HUR, PI13, PI, hea; Clonality Polyclonal; Research Area Proteolysis / Ubiquitin; Add to Compare List. Local … punalippuoireitaWebHuR inhibitor KH-3 is a small molecule, selective inhibitior of HuR function with Ki of 0.83 and 0.72 uM in FP and AlphaLISA assays, respectively. KH-3 potently inhibits breast cancer cell growth in vitro and in vivo. KH-3 inhibits breast cancer cell invasion in vitro as well as delays initiation of lung colonies and improves mouse survival in ... punalevätpunalippuoireet