WebProduct Details Product Specific Information This antibody detects pS, pT and pY amino acids with very low cross reactivity to their non-phosphorylated variants Target Information There are three amino acids … Web10 feb. 2011 · Using a phospho-Ser 318-specific HuR antibody, we found a strong increase in nuclear HuR phosphorylation in DLD-1 cells when compared with nontransformed CCD 841 colon epithelial cells. Importantly, a strong increase in HuR phosphorylation at Ser 318 was also found in tissue specimen from human colon carcinomas.
HuR Posttranscriptionally Regulates WEE1: Implications for the …
Web22 dec. 2014 · The mRNA binding protein HuR is over expressed in cancer cells and contributes to disease progression through post-transcriptional regulation of mRNA. The regulation of HuR and how this relates to glioma is the focus of this report. SRC and c-Abl kinases regulate HuR sub-cellular trafficking and influence accumulation in the … Web14 mrt. 2024 · To determine the MUC16 mediated HuR target genes in TNBC, we performed RNA immunoprecipitation (RIP) with HuR antibody, followed by a tumor metastasis PCR array. First, HuR target genes were pull-down using HuR antibody from scramble control (HCC1806-SCR) cells and MUC16 knockdown (HCC1806-shMUC16) … punalakintie 9
High-constitutive HuR phosphorylation at Ser 318 by PKC{delta ... - PubMed
WebImmunocytochemistry/ Immunofluorescence - Anti-HuR / ELAVL1 antibody (ab196626) Immunofluorscence analysis of MCF7 cells labeling HuR / ELAVL1 with ab196626 at 1/100 dilution (top panel). Blue: DAPI for nuclear staining (bottom panel). Western blot - Anti-HuR / ELAVL1 antibody (ab196626) WebB.RIP Lysate prepared from HeLa cells (0.5 × 10 6 or 1.7 × 10 6 cell equivalents per IP) were immunoprecipated using 2.5 μg of either a normal Rabbit IgG or Anti-HUR antibody and the Imprint RNA Immunoprecipitation Kit . Immunoprecipitation of HUR-target and non-target associated RNA was validated by qPCR using Control Primers Actin B and JUN. WebUsing a phospho-Ser 318-specific HuR antibody, we found a strong increase in nuclear HuR phosphorylation in DLD-1 cells when compared with nontransformed CCD 841 colon epithelial cells. Importantly, a strong increase in HuR phosphorylation at Ser 318 was also found in tissue specimen from human colon carcinomas. punakylkirastas ääni