Myotube fusion index
WebMyotube fusion index was determined by counting the nuclei in every myotube (defined as MyHC‐positive cells containing ≥2 nuclei) per field and dividing by the total number of nuclei in the field. Images were analyzed using ImageJ, which was downloaded from the National Institutes of Health website (Bethesda, MD, USA) [ 15 ]. WebJun 22, 2024 · In 2D, enhanced myogenesis was observed, as evidenced by an increased myotube diameter and fusion index. Second, 2D results were translated towards 3D …
Myotube fusion index
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WebMar 31, 2024 · The final ATAC and gene expression libraries were sequenced on an Illumina Novaseq 6000 with v1.5 Kit. For the ATAC libraries, 24 bp of cell barcode and spacer, 8 bp single index read, and 100 bp paired-end reads were generated; for the gene expression libraries, 100 bp paired-end reads and 10 + 10 index reads were generated. WebApr 19, 2024 · High concentrations of arachidonic acid have a deleterious effect on both myoblast proliferation, myotube area, and myotube fusion. Our data demonstrates that arachidonic acid reduced proliferation by 85% and 32%, myotube area by 63% and 38% and myotube fusion index by 93% and 50% respectively (P<0.001 for all).
WebDec 20, 2024 · A fusion index demonstrated that such scDKO myoblasts exhibited a significant defect in ERKi-induced secondary fusion compared with those isolated from their WT littermates (Figures 6 E and 6F). Specifically, scDKO myoblasts exhibited a loss of the hyperfused myotubes observed in the WT cultures and instead accumulated … WebJan 24, 2024 · Our study shows that Piezo1 is essential for terminal muscle differentiation acting on myoblast fusion, suggesting that Piezo1 deregulation may have implications in muscle aging and degenerative diseases, including muscular dystrophies and neuromuscular disorders.
WebNational Center for Biotechnology Information WebApr 11, 2024 · In contrast to the proliferation behavior indicated in Fig. 1d, the experimental groups (G2 and G3 with low FBS content) exhibit a high fusion index of 55.6% and 58.8%, respectively, while the ...
WebJun 28, 2024 · Myotube diameter and nuclear fusion index (NFI). The effect of ex vivo human serum-conditioned media on myotube diameter and NFI in vitro. A: representative images illustrating atrophy in myotubes conditioned with serum from older males in comparison to untreated control C2C12 myotubes and myotubes treated with serum from …
WebJan 24, 2024 · By this point, myoblasts seeded at high confluency began to show myotube formation in the relatively small 96-well plates. These early formed myotubes were then administered Yoda1. Yoda1 binds the agonist transduction motif (ATM), located at the pore domain of the Piezo1 channel [ 17 ]. chronic kidney disease stage 2 gfr 60-89WebJan 28, 2024 · The Fusion index was calculated by counting the number of nuclei in myotubes containing more than 2 nuclei divided by the total number of nuclei from the same field. 2.7. Bioenergetics Assay C2C12 cells were incubated with DHA, AA or BSA for 48 h to differentiate to myotubes. chronic kidney disease stage 3-4 icd 10WebAdditionally, immunofluorescence staining of MyHC and MyoD shows that overexpression of miR-424(322)-5p reduces the number of myotubes and decreases the myotube fusion index. Consistently, inhibition of its function mediated by an inhibitor promotes the expressions of myogenic markers and myotube fusion. chronic kidney disease stage 3 aafpWeb例文 Myotube area and fusion index were measured to quantify myotube differentiation. 筋管面積および融合指数を測定して、筋管分化を定量化した。 例文 Myoblast proliferation and myotube formation are critical early events in skeletal muscle regeneration. chronic kidney disease stage 2aWebJan 1, 2013 · 3.1 The Effect of Taurine Treatment on the Differentiation of C2C12 Myoblast to Myotube. The effect of taurine treatment on the differentiation of myoblast to myotube in C2C12 cells was evaluated by the measurements of maximal length of short diameter of differentiated myotube, the number of nucleus in the myotube, and the calculation of … chronic kidney disease stage 2 diet listWebA syncytial myotube is obtained by one FC fusing with a determined number of FCMs to generate a muscle displaying a specific number of nuclei. The fusion process occurs … chronic kidney disease stage 2 mild symptomsWebJan 21, 2024 · MyoCount, a new open-source program developed by Murphy et al., is a promising new tool that can analyze myotube morphology and nuclear fusion index. It … chronic kidney disease stage 3 a