WebJun 10, 2024 · For each sample, SEAP concentration was assessed in 50 μl aliquots of culture supernatant, with CPSD as an indicator substrate (Tropix Phospha-Light Chemiluminescent Assay Kit, Life Technologies, catalog no. T1017), using a minor variant of the manufacturer’s instructions; luminescence intensity was measured using a Varioskan … WebThe NovaBright™ Phospha-Light™ EXP Assay Kit is a second generation chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase (SEAP) or the non-secreted placental isozyme PLAP. This assay combines ultrasensitive detection with a dynamic range of 5 orders of magnitude.
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Webtase using the Phospha-Light System (Life Technologies). Bioinformatics TargetScan Human Custom release 5.2 (29) was used toidentifyconserved canonical binding sites for the hcmv-miR-UL22A-5p seed region (nt 2–8). MRNA–miRNA interactions were predicted using RNAhybrid (30) with WebAug 31, 2024 · ALG-2 is a penta-EF-hand Ca2+-binding protein and interacts with a variety of proteins in mammalian cells. In order to find new ALG-2-binding partners, we searched a human protein database and retrieved sequences containing the previously identified ALG-2-binding motif type 2 (ABM-2). After selecting 12 high-scored sequences, we expressed … notebook cases 10 inch
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WebPhospha-Light™ assay system is a chemiluminescent reporter gene assay for the sensitive detection of secreted human placental alkaline phosphatase used as a... Shop. English Change country. Get All The Latest News Help & Support Center › English Change country; 0. No offer available ... Webt1015 phospha-light (200 assays) each 2237.2. t1016 phospha-light 10000 assay each 33636.4. t1017 phospha-light (600 assays) each 4480. t1018 phospha-light hgs-4x each 11419.8. t10182 tetramethylrhodamine azide 0.5 mg 2460.5. t10183 tetramethylrhodamine (tamra) 0.5 mg 2460.5. t1019 galacto-star yeast 3kit 200 assay 2424.1. t1020 ... Websystem kit (Phospha-Light System; Applied Biosys-tems, Foster City, CA). After incubation of mixtures of the medium supernatant and kit components on a white 96-well microplate for 4–20min, the plate was placed in a plate luminometer (1420 ARVOsx Multilabel Counter; PerkinElmer, Waltham, MA), and the luminescence in how to set lockscreen as slideshow